PIPRA

Pierce's Disease Research Symposium Proceedings from 2003

• Epidemiology Of Pierce’S Disease In The Central San Joaquin Valley Of California: Factors Affecting Pathogen Distribution And Movement
  Project Leaders: Russell Groves Jianchi Chen USDA-ARS-SJVASC Parlier, CA 93648 Cooperators: Kent Daane Division of Insect Biology University of California Berkeley, CA 94720 Marshall Johnson Department of Entomology University of California Riverside, CA 92521


• Monitoring And Control Measures For Pierce’S Disease In Kern County, And Epidemiological Assessments Of Pierce’S Disease
  Project Leaders: Jennifer Hashim UC Cooperative Extension Bakersfield, CA 93307 Cooperators: Maggi Kelly & David Shari Center for the Assessment and Monitoring of Forest and Environmental Resources University of California Berkeley, CA 94720 Barry L. Hill Calif. Dept. of Food & Agriculture Sacramento, CA 95814 Alexander H. Purcell Department of. Environmental Science Policy and Management University of California Berkeley, CA 94720


• Characterize And Assess The Biocontrol Potential Of Bacterial Endophytes Of Grapevines In California
  Project Leader: Bruce Kirkpatrick, Department of Plant Pathology University of California Davis, CA 95616 Cooperators: Dawna-Darjean Jones Department of Plant Pathology University of California, Davis Davis, CA 95616 Edwin Civerolo USDA-ARS-PWA SJV Agriculture Science Center Parlier, CA A.H. Purcell Division of Insect Biology University of California Berkeley, CA 94720


• Evaluation Of Bactericides And Modes Of Delivery For Managing Pierce'S Disease
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Cooperators: Chad Vargas Dawna-Darjean Jones Dept. of Plant Pathology University of California Davis, CA 95616 Peter Andersen University of Florida Quincy, FL 32351 Edwin Civerolo USDA-ARS Parlier, CA A.H. Purcell Division of Insect Biology University of California Berkeley, CA Rhonda Smith UC Cooperative Extension Sonoma, CA


• Treatment Thresholds For The Glassy-Winged Sharpshooter Based On The Local Epidemiology Of Pierce’S Disease Spread
  Project Leader: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Cooperators: Charles A. Farrar, Rayda K. Krell, and Yong-Lak Park Department of Entomology University of California Riverside, CA 92521


• Epidemiology Of Pierce'S Disease In The Coachella Valley
  Project Leader: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Cooperators: Charles A. Farrar and Rayda Krell Department of Entomology University of California Riverside, CA


• Fate Of Xylella Fastidiosa In Alternate Hosts
  Project Leader: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720 Research Associate: Christina Wistrom Division of Insect Biology University of California Berkeley, CA 94720


• Characterization And Studies On The Fundamental Mechanisms Of Xylella Fastidiosa Transmission To Grapevines By The Glassy-Winged Sharpshooter
  Project Leader: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720-3112 Cooperators: Elaine Backus USDA-ARS San Joaquin Valley Agric. Sci. Ctr. Parlier, CA 93648 Researcher: Rodrigo P.P. Almeida Department of Plant and Environmental Protection Sciences University of Hawaii at Manoa Honolulu, HI 96822 Jennifer Hashim UC Cooperative Extension Bakersfield, CA 93307 Barry Hill CDFA, Plant Pest Diagnostics Branch Sacramento, CA 95832-1448


• Glassy-Winged Sharpshooter Transmission Of Xylella Fastidiosa To Almond
  Project Leader: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720-3112 Researcher: Rodrigo P.P. Almeida Department of Plant and Environmental Protection Sciences University of Hawaii at Manoa Honolulu, HI 96822


• Xylem Chemistry Mediation Of Resistance To Pierce’S Disease
  Project Leader: Peter C. Andersen University of Florida, NFREC Quincy, FL 32351 Cooperators: Breno Leite University of Florida, NFREC Quincy, FL 32351 Maria Lucia Ishida University of Florida, NFREC Quincy, FL 32351


• Documentation And Characterization Of Xylella Fastidiosa Strains In Landscape Hosts
  Project Leader: Frank Wong Department of Plant Pathology University of California Riverside, CA 92521 Cooperators: James Downer UC Cooperative Extension Ventura County John Karlik UC Cooperative Extension Kern County Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521 Mike Henry UC Cooperative Extension Riverside County Michelle LeStrange UC Cooperative Extension Tulare County Heather S. Costa Department of Entomology University of California Riverside, CA 92521 John Kabashima UC Cooperative Extension Orange County David Shaw UC Cooperative Extension San Diego County


• Sharpshooter Feeding Behavior In Relation To Transmission Of The Pierce’S Disease Bacterium
  Project Leader: Elaine Backus USDA-ARS-PWA Exotic & Invasive Dis. & Pests Parlier, CA 93648 Cooperators: Matthew Blua Department of Entomology University of California Riverside, CA 92521 Personnel: Fengming Yan College of Life Sciences Peking University Beijing, China Alexander Purcell Division of Insect Biology University of California Berkeley, CA 92521 Javad Habibi Department of Entomology University of Missouri Columbia, MO 65211 William Bennett Private consultant 744 Elktown Rd. Otterville,MO 65348


• Paratransgenesis To Control Pierce'S Disease: Biology Of Endophytic Bacteria In Grape Plants And Bioassay Of Reagents To Disrupt Pierce’S Disease
  Project Leader: Blake Bextine Department of Entomology University of California Riverside, 92521 Collaborators: Carol Lauzon Department of Biological Sciences California State University, Hayward Hayward, CA 94542 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, 92521 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Consultant: Frank Richards Yale Medical School New Haven, CT 06520


• Roles Of Xylella Fastidiosa Proteins In Virulence
  Project Leader: George Bruening Department of Plant Pathology University of California, Davis 95616 Cooperators: Edwin Civerolo San Joaquin Valley Agricultural Sciences Center Exotic and Invasive Diseases and Pest Research USDA-ARS-PWA, Parlier, CA Abhaya M. Dandekar Department of Pomology Univ. of California, Davis, CA Goutam Gupta MS M888 Biology Division Los Alamos Nat. Lab.


• Control Of Pierce’S Disease Through Degradation Of Xanthan Gum
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California Riverside 92521 Cooperator: Neal L. Schiller Division of Biomedical Sciences University of California Riverside, CA 92521


• Culture-Independent Analysis Of Endophytic Microbial Communities In Grapevine In Relation To Pierce’S Disease
  Project Leaders: Donald A. Cooksey and James Borneman Department of Plant Pathology University of California Riverside 92521


• Insect-Symbiotic Bacteria Inhibitory To Xylella Fastidiosa In Sharpshooters: Toxic Peptides Against Xylella
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521 Collaborators: David Lampe Biology Department Duquesne University Pittsburgh, PA 19219 Project Director: Thomas Miller Department of Entomology University of California Riverside, CA 92521 Carol Lauzon Dept. of Biological Sciences California state University Hayward, CA 94542 Blake Bextine Dept. of Entomology University of California Riverside, 92521


• Impact Of Multiple-Strain Infections Of Xylella Fastidiosa On Acquisition And Transmission By The Glassy-Winged Sharpshooter
  Project Leaders: Heather S. Costa Department of Entomology University of California Riverside, CA 92521 Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521


• Rapid Screening Of Grape Cdna Libraries And Functional Testing Of Genes Conferring Resistance To Pierce’S Disease
  Project Leaders: David Gilchrist Department of Plant Pathology University of California Davis, CA 95616 Cooperators: Andrew Walker Department of Viticulture and Enology University of California Davis CA 95616 James E. Lincoln Department of Plant Pathology University of California Davis, CA 95616 Steven Lindow Department of Plant and Microbial Biology University of California Davis, CA 95616


• Biological, Cultural, Genetic, And Chemical Control Of Pierce'S Disease: Significance Of Riparian Plants In The Epidemiology Of Pierce’S Disease
  Project Leader: Kendra Baumgartner USDA-ARS Davis, CA 95616 Cooperators: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720 M. Andrew Walker Dept. of Viticulture and Enology University of California Davis, CA 95616


• The Development Of Pierce’S Disease In Xylem: The Roles Of Vessel Cavitation, Cell Wall Metabolism And Vessel Occlusion
  Project Leaders: John Labavitch Department of Pomology University of California Davis, CA 95616 Cooperators: L. Carl Greve Department of Pomology University of California, Davis, CA 95616 Eleanor Thorne Department of Viticulture and Enology University of California Davis, CA 95616 Tom Rost Section of Plant Biology University of California Davis, CA 95616 Caroline Roper Department of Plant Pathology University of California Davis, CA 95616


• Paratransgenesis To Control Pierce’S Disease: Isolation And Analysis Of Anti-Xylella Single Chain Antibodies
  Project Leader: David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19282 Collaborators: Carol Lauzon Dept. of Biological Sciences California State University, Hayward Hayward, CA 94542 Blake Bextine Dept. of Entomology University of California Riverside, 92521 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, 92521 Don Cooksey Dept. of Plant Pathology University of California Riverside, 92521 Frank Richards Yale Medical School New Haven, CT 06520


• Paratransgenesis To Control Pierce’S Disease: The “Social Life” Of Alcaligenes Xylosoxidans Denitrificans
  Project Leaders: Carol Lauzon Department of Biological Sciences California State University, Hayward Hayward, CA 94542 Collaborators: Blake Bextine Department of Entomology University of California, Riverside, 92521 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Thomas A. Miller Department of Entomology University of California Riverside, 92521 Don Cooksey Department of Plant Pathology University of California Riverside, 92521 Frank Richards Yale Medical School New Haven, CT 06520


• Characterization And Identification Pierce’S Disease Resistance Mechanisms: Analysis Of Xylem Anatomical Structures And Of Natural Products In Xylem Sap Among Vitis
  Project Leader: Hong Lin Exotic & Invasive Disease & Pest Research Unit USDA-ARS Parlier, CA 93648 Cooperator: Andrew Walker Department of Viticulture and Enology University of California Davis, CA, 95616


• Differential Plant-Inducibility Of Putative Virulence Genes By Xylella Fastidiosa In Susceptible And Resistant Plant Hosts
  Project Leader: Steven E. Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperator: Karyn Lynn Newman Department of Plant and Microbial Biology University of California Berkeley, CA 94720


• Exploration For Facultative Endosymbionts Of Sharpshooters
  Project Leader: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720 Cooperators: Eoin Brodie (laboratory of Mary Firestone) and George Roderick Dept. of Environmental Science, Policy and Management University of California, Berkeley, CA Researcher: Clytia Montllor Curley University of California Division of Insect Biology Berkeley, CA 94720-3112 Chris Carlton Dept. of Entomology Louisiana State University Baton Rouge, LA 70803 Russell Mizell University of Florida NFREC Monticello, FL 32351-5677


• Role Of Bacterial Attachment In Transmission Of Xylella Fastidiosa By The Glassy-Winged Sharpshooter,And Other Factors Affecting Transmission Efficiency
  Project Leader: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720-3112 Cooperators: Steve Lindow Plant and Molecular Biology 111 Koshland Hall University of California Berkeley, CA 94720-3102 Researcher: Rodrigo P.P. Almeida Department of Plant and Environmental Protection Sciences University of Hawaii at Manoa Honolulu, HI 96822 Karyn Newman Plant and Molecular Biology 111 Koshland Hall University of California Berkeley, CA 94720-3102


• Mechanisms Of Pierce’S Disease Transmission In Grapevines: Relevance Of Hydraulic Architecture
  Project Leaders: Thomas L. Rost Section of Plant Biology University of California Davis, CA 95616 Mark A. Matthews Dept of Viticulture and Enology University of California Davis, CA 95616 Joshua F. Stevenson Section of Plant Biology University of California Davis, CA 95616


• Characterization Of Resistance To Pierce’S Disease In Muscadinia Rotundifolia And The Potential For Conferring Resistance To Grafted Vitis Vinifera Scions
  Project Leaders: Andrew Walker Jonathan Ruel Department of Viticulture and Enology University of California Davis, CA 95616


• Pressure Chamber Extraction Of Xylem Fluid: Improving Bacterial Detection In Plants Affected By Xylella Fastidiosa
  Project Leader: Blake Bextine Department of Entomology University of California Riverside, CA 92521 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521


• Developing A Method To Detect Xylella Fastidiosa In The Glassy-Winged Sharpshooter
  Project Leaders: Blake Bextine Matthew J. Blua Richard Redak Department of Entomology University of California Riverside, CA 92521


• Relationships Between Total Population Counts Of Glassy-Winged Sharpshooters And Numbers Obtained From Various Sampling Methods
  Project Leaders: Matthew J. Blua Richard Redak Department of Entomology University of California Riverside Riverside, CA 92521 Cooperators: David H. Akey USDA-ARS Cotton Research Laboratory Phoenix, AZ Carlos E. Coviella Department of Entomology UC Riverside Riverside, CA 92521


• A Monoclonal Antibody Specific To Glassy-Winged Sharpshooter Egg Protein: A Tool For Predator Gut Analysis
  Project Leaders: James Hagler Western Cotton Research Lab USDA-ARS Phoenix, AZ 85040 Cooperators: Valerie Fournier Division of Insect Biology University of California Berkeley, CA 94720 Russell Grove Exotic & Invasive Dis. & Pests Lab USDA-ARS Parlier, CA 93648 Kent Daane Division of Insect Biology University of California Berkeley, CA 94720 David Akey Western Cotton Research Lab USDA-ARS Phoenix, AZ 85040 Jesus de Leon Beneficial Insects Research Unit USDA-ARS Weslaco, TX 78596 Heather Costa Department of Entomology University of California


• Functional Genomics Of The Grape-Xylella Interaction: Towards The Identification Of Host Resistance Determinants (Cumulative Progress Report)
  Project Leaders: Douglas R. Cook Francisco Goes-da-Silva Hyunju Lim Department of Plant Pathology University of California Davis, CA 95616 Cooperators: M. Andrew Walker Alberto Iandolino Department of Viticulture and Enology University of California Davis, CA 95616


• Development Of Trapping Systems To Trap Glassy-Winged Sharpshooter (Homalodisca Coagulata) Adults And Nymphs In Grape
  Project Leader: Raymond L. Hix Department of Entomology University of California Riverside, CA 92521 Cooperators: Michael R. McGuire Western Integrated Cropping Systems USDA-ARS Shafter, CA 93263 Gary Puterka USDA-ARS Appalachian Fruit Research Station Kearneysville, WV


• Sampling, Seasonal Abundance, And Comparative Dispersal Of Glassy-Winged Sharpshooters In Citrus And Grapes: Sampling Progress Report
  Project Leaders: Steve Naranjo Steven Castle USDA-ARS Phoenix, AZ Nick Toscano Department of Entomology UC Riverside


• Rapid Bioluminescent Monitoring Of Infection By The Pierce’S Disease Agent
  Project Leader: Glenn M. Young Department of Food Science and Technology University of California Davis, CA 95616 Cooperator: John Labavitch Department of Pomology University of California Davis, CA 95616


• Sampling, Seasonal Abundance, And Comparative Dispersal Of Glassy-Winged Sharpshooters In Citrus And Grapes: Dispersal Progress Report
  Project Leaders: Jacquelyn L. Blackmer James R. Hagler WCRL-USDA-ARS Phoenix, AZ 85040


• Population Genetic Structure Of The Glassy-Winged Sharpshooter Determined By Issr-Pcr Dna Fingerprinting
  Project Leader: Jesse H. de León USDA- ARS Beneficial Insects Research Unit Weslaco, Texas 78596 Cooperators: Walker A. Jones USDA- ARS Beneficial Insects Research Unit Weslaco, Texas 78596 David J. W. Morgan Cal. Dept. Food and Agriculture Mount Rubidoux Field Station Riverside, California 92501


• Ultrastructural Contributions To The Study Of The Glassy-Winged Sharpshooter And Pierce’S Disease
  Project Leader: Thomas P. Freeman Electron Microscopy Laboratory North Dakota State University Fargo, ND 58105 Cooperators: Roger A. Leopold Dennis R. Nelson James S. Buckner USDA-ARS Biosciences Research Laboratory Fargo, ND 58105 Thomas J. Henneberry Western Cotton Research Laboratory Phoenix, AZ 85040


• Lrec
  Experiment – Navels The ground around 45 individual trees of block 23 at the Lindcove Field Station were treated by backpack sprayer with the equivalent of 32 oz of Admire per acre on 30 Apr 2002 and 20 Jun 2003. An equal number of trees were left untreated. Before treatment, the 90 trees were chosen based on similar yields (1 March 2002). After the first treatment (11 February 2003), fruit were harvested and evaluated for number, size and quality. There were no significant differences in any of the parameters tested for the treated and untreated trees.


• Rootstock Variety Influence On Pierce’S Disease Symptoms In Grafted Chardonnay And Cabernet Sauvignon (Vitis Vinifera L.) Grapevines
  Project Leaders: Peter Cousins Plant Genetic Resources Unit, USDA-ARS New York State Agricultural Experiment Station Geneva, NY 14456 Cooperators: Zhongbo Ren Center for Viticulture and Small Fruit Research Florida A&M; University Tallahassee, FL


• Developing A Stable Classification Of The Glassy-Winged Sharpshooter Genus Homalodisca
  Project Leader: Stuart H. McKamey Systematic Entomology Laboratory PSI, Agricultural Research Service U. S. Department of Agriculture c/o National Museum of Natural History Smithsonian Institution Washington, DC 20560-0168 Cooperators: Roman Rakitov Center for Biodiversity Illinois Natural History Survey Champaign, IL 61820 Daniela Takiya Center for Biodiversity Illinois Natural History Survey 607 E. Peabody Dr. Champaign, IL 61820


• Developing Transcriptional Profiles And Microarray Expression Analysis Of Grape Plant Response To Xylella Fastidiosa
  Project Leader: Hong Lin Exotic & Invasive Disease & Pest Research Unit USDA/ARS Parlier, CA 93648 Cooperators: Andrew Walker Department of Viticulture and Enology University of California Davis, CA, 95616


• Keys To Management Of The Glassy-Winged Sharpshooter: Interactions Between Host Plants, Malnutrition, And Natural Enemies
  Project Leaders: Russell F. Mizell, III Peter C. Andersen University of Florida, NFREC-Quincy Quincy, FL 32351


• Field Evaluation Of Grape Rootstock Response To Natural Infection By Pierce’S Disease
  Project Leaders: Jiang Lu Zhongbo Ren Center for Viticulture and Small Fruit Research Florida A&M; University Tallahassee, Florida 32317 Peter Cousins Plant Genetic Resources Unit, USDA-ARS New York State Agricultural Experiment Station Geneva, New York


• Area-Wide Management Of The Glassy-Winged Sharpshooter In The Temecula And Coachella Valleys
  Project Leaders: Raymond L. Hix Department of Entomology University of California Riverside, CA 92521 Nick C. Toscano Department of Entomology University of California Riverside, CA 92521 Carmen Gispert UC Cooperative Extension Riverside County


• Development Of Peptide Antibiotic-Based Control Strategies For Xylella Fastidiosa
  Project Leaders: Shizuo George Kamita Department of Entomology University of California Davis, CA 95616 Cooperators: David G. Gilchrist Department of Plant Pathology University of California Davis, CA 95616 Bruce D. Hammock Department of Entomology University of California Davis, CA 95616 Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616


• Toward A Standardized Treatment Protocol To Eliminate Glassy-Winged Sharpshooter Egg Masses In Commercial Nursery Stock
  Project Leaders: Richard Redak Department of Entomology University of California Riverside, CA 92521 Cooperators: James Bethke Department of Entomology University of California Riverside, CA 92521


• Impact Of Layering Control Tactics On The Spread Of Pierce'S Disease By The Glassy-Winged Sharpshooter
  Project Leaders: Richard Redak Department of Entomology University of California Riverside, CA 92521 Matthew J. Blua Department of Entomology University of California Riverside, CA 92521


• Laboratory And Field Evaluations Of Imidacloprid, Thiamethoxam, And Acetamiprid Against The Glassy-Winged Sharpshooter On Grapevines
  Project Leaders: Nick C. Toscano Dept of Entomology University of California Riverside, CA 92521 Cooperators: Jianlong Bi Dept of Entomology University of California Riverside, CA 92521 Frank J. Byrne Dept of Entomology University of California Riverside, CA 92521 Nilima Prabhaker Dept of Entomology University of California Riverside, CA 92521 Steven J. Castle Western Cotton Research Laboratory USDA-ARS Phoenix, AZ Mac Learned Bayer CropScience Paso Robles, CA Carmen Gispert UC Cooperative Extension Riverside County Riverside, CA


• Directing Potential Anti-Xylella Gene Products To The Xylem Of Transgenic Grapevines
  Project Leaders: Carole Meredith Department of Viticulture and Enology University of California Davis, 95616 Cooperators: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Abhaya Dandekar Department of Pomology University of California Davis, 95616 John Labavitch Department of Pomology University of California Davis, CA 95616


• Genome-Wide Identification Of Rapidly Evolving Genes In Xylella Fastidiosa: Key Elements In The Systematic Identification Of Host Strains, And In The Search For Plant-Host Pathogenicity Candidate Genes
  Project Leader: Leonard Nunney Department of Biology University of California Riverside, CA 92521 Cooperators: Richard Stouthamer Robert Luck Department of Entomology University of California Riverside, CA 92521


• Testing Transgenic Grapevines For Resistance To Pierce’S Disease
  Project Leaders: Bruce I. Reisch Department of Horticultural Sciences NYS Agricultural Experiment Station Cornell University Geneva, NY 14456 Julie R. Kikkert Department of Horticultural Sciences NYS Agricultural Experiment Station Cornell University Geneva, NY 14456 Cooperator: José Vidal NYS Agricultural Experiment Station Cornell University Geneva, NY 14456


• Magnetic Resonance Imaging: A Non-Destructive Approach For Detection Ofxylem Blockages In X. Fastidiosa-Infected Grapevines
  Project Leaders: Ken Shackel John Labavitch Department of Pomology University of California Davis, CA 95616 Cooperators: Mark Matthews Department of Viticulture and Enology University of California Davis, CA 95616 L. Carl Greve Department of Pomology University of California Davis, CA 95616 Jeffrey Walton NMR Facility University of California Davis, CA 95616 Alonso Perez Department of Pomology University of California Davis, CA 95616


• Marker-Assisted Selection For Resistance To Xylella Fastidiosa: Accelerated Breeding Of Pierce’S Disease Resistant Grapes
  Project Leaders: Andrew Walker, Alan Krivanek, and Summaira Riaz Department of Viticulture and Enology University of California Davis, CA 95616


• Map Based Identification And Positional Cloning Of Xylella Fastidiosa Resistance Genes From Known Sources Of Pierce’S Disease Resistance In Grape
  Project Leaders: Andrew Walker and Summaira Riaz Department of Viticulture and Enology University of California Davis, CA 95616


• The Genetics Of And Breeding For Pierce’S Disease Resistant Grapes
  Project Leaders: Andrew Walker & Alan Tenscher Department of Viticulture and Enology University of California Davis, CA 95616 David Ramming USDA-ARS 9611 South Riverbend Ave. Parlier, CA 93648


• Real Time Pcr For Clinical Detection And Differentiation Of Xylella Fastidosa Strains M.
  Francis Department of Plant Pathology University of California Davis, CA 95616 E.L. Civerolo and H.L. Lin USDA-ARS-PWA San Joaquin Valley Agriculture Science Center Parlier, CA 93648


• Real Time Pcr For Clinical Detection And Differentiation Of Xylella Fastidosa Strains M.
  Francis Department of Plant Pathology University of California Davis, CA 95616 E.L. Civerolo and H.L. Lin USDA-ARS-PWA San Joaquin Valley Agriculture Science Center Parlier, CA 93648


• Developing A Microarray-Pcr-Based Identification And Detection System For Xylella Fastidiosa Strains Important To California
  Project Leader: Jianchi Chen USDA-ARS-PWA San Joaquin Valley Agricultural Sciences Center Exotic and Invasive Diseases and Pests Research Parlier, CA 93648 Cooperator: Edwin L. Civerolo USDA-ARS-PWA San Joaquin Valley Agricultural Sciences Center Exotic and Invasive Diseases and Pests Research Parlier, CA 93648


• Dna Microarray And Mutational Analysis To Identify Virulence Genes In Xylella Fastidiosa
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521 Cooperator: Ching-Hong Yang Department of Biology University of Wisconsin-Milwaukee


• Understanding Xylella Fastidiosa Colonization And Communication In Xylem Lumina
  Project Leaders: Harvey C. Hoch Thomas J. Burr Department of Plant Pathology Cornell University NYSAES Geneva, New York 14456 Post-doctoral Researcher: Yizhi Meng Department of Plant Pathology Cornell University NYSAES Geneva, New York 14456 Cooperator: James N. Turner New York State Department of Health Wadsworth Center Albany, New York


• The Xylella Fastidiosa Cell Surface
  Project Leader: Michele M. Igo Section of Microbiology Division of Biological Sciences University of California, Davis Davis, CA 95616 Cooperators: Bruce Kirkpatrick Department of Plant Pathology University of California, Davis Davis, CA 95616 M. Andrew Walker Department of Viticulture and Enology University of California, Davis Davis, CA 95616


• Analysis Of Xylella Fastidiosa Transposon Mutants And Development Of An En Planta Xylella Fastidiosa Plasmid Vector
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California, Davis Davis, CA 95616 Cooperator: Magalie Guilhabert Department of Plant Pathology University of California, Davis Davis, CA 95616


• Management Of Pierce’S Disease Of Grape By Interfering With Cell-Cell Communication In Xylella Fastidiosa
  Project Leader: Steven E. Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Karyn Lynn Newman Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Alexander Purcell Rodrigo Almeida Department of Environmental Science Policy and Management University of California Berkeley, CA 94720


• Role Of Attachment Of Xylella Fastidiosa To Grape And Insects In Its Virulence And Transmissibility
  Project Leader: Steven E. Lindow University of California Department of Plant and Microbial Biology Berkeley, CA 94720-3112 Cooperator: Alexander H. Purcell University of California Division of Insect Biology Berkeley, CA 94720-3112


• Determination Of Genes Conferring Host Specificity In Grape Strains Of Xylella Fastidiosa Using Whole-Genomic Dna Microarrays
  Project Leaders: Steven E. Lindow Department of Plant and Microbial Biology 111 Koshland Hall University of California Berkeley, CA 94720 Paul Richardson Head-Genomic Technologies DOE, Joint Genome Institute 2800 Mitchell Drive, B400 Walnut Creek, CA 94598


• Surrogate Genetics For Xylella Fastidiosa
  Project Leader: Valley Stewart Section of Microbiology Division of Biological Sciences University of California Davis, CA 95616 Cooperators: Michele Igo Section of Microbiology Division of Biological Sciences University of California Davis, CA 95616 Bruce Kirkpatrick Department of Plant Pathology College of Agricultural & Environmental Sciences University of California Davis, CA 95616


• Environmental Fate Of A Genetically Marked Endophyte In Grapevines And The Glassy-Winged Sharpshooter
  Project Leader: Blake Bextine Department of Entomology University of California, Riverside, 92521 Project Director: Thomas A. Miller Department of Entomology University of California, Riverside, 92521 Collaborators: Carol Lauzon Department of Biological Sciences California State University, Hayward Hayward, CA 94542 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Consultant: Frank Richards Yale University New Haven, CT 06520


• Biological Control Of Pierce’S Disease With Non-Pathogenic Strains Of Xylella Fastidiosa
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California, Riverside 92521


• Epidemiology Of Pierce’S Disease In Southern California: Identifying Inoculum Sources And Transmission Pathways
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California, Riverside 92521


• Design Of Chimeric Anti-Microbial Proteins For Rapid Clearance Of Xylella
  Project Leader: Abhaya M. Dandekar Department of Pomology University of California Davis, CA 95616 Elizabeth Hong-Geller B-1, MS M888, LANL Los Alamos, NM 87545 Cooperators: George Bruening Department of Plant Pathology University of California Davis, CA 95616 Pat J. Unkefer B-3, MS E529, LANL Los Alamos, NM 87545 Nam Goutam Gupta B-1, MS M888,LANL Los Alamos, NM87545 Karen McDonald Chem Engg & Material Sciences University of California Davis, CA 95616 Edwin L. Civerolo SJVASC Parlier, CA 93468 Cliff J. Unkefer B-3, MS G758, LANL Los Alamos, NM 87545 Patrick R. Shiflett MS M888,B Division, LANL Los Alamos, NM 87545


• Role Of Type I Secretion In Pierce’S Disease
  Project Leader: Dean W. Gabriel Department of Plant Pathology University of Florida Gainesville, FL 32611 Cooperator: Richard Lee Citrus Research and Education Center University of Florida Lake Alfred, FL